Invitrogen™ IFN gamma Human ELISA Kit

Measure levels of human interferon gamma (IFNg) in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples using 96-well plates.

Catalog Number	Mfr. No.	Description	Sufficient For
ENEHIFNG	Invitrogen™ EHIFNG	Human IFN gamma ELISA Kit	96 microplate wells
ENEHIFNG2	Invitrogen™ EHIFNG2	Human IFN gamma ELISA Kit	2 x 96 microplate wells
ENEHIFNG5	Invitrogen™ EHIFNG5	Human IFN gamma ELISA Kit	5 x 96 microplate wells

Description

Assay IFN-g (human interferon gamma) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Invitrgen™ Human IFN gamma ELISA Kits.

These human IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IFNγ antibody pairs and IFNγ standard only. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant human interferon gamma (IFNγ)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 25.6 to 1000pg/mL

• Sensitivity: ≤ 2pg/mL

• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

• Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 3.5 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-6, IL-7, IL-8, TNFα or GM-CSF

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Specifications

• Accession Number: P01579
• Assay Sensitivity: <2 pg/mL
• Conjugate: Biotin
• Product Type: ELISA Kit
• Specificity: No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, TNFa or GM-CSF
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IFG,IFI
• Intraassay CV: <10%
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 25.6-1000 pg/mL
• Description: Human IFN gamma ELISA Kit
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• Gene ID (Entrez): 3458
• Interassay CV: <12%
• Label or Dye: HRP
• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Target: Human
• Total Assay Time: 3 hr.