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Gibco™ Human IGF-I Recombinant Protein

Catalog No. PHG0078
Encompass_Preferred
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Quantity:
100 μg
20 μg

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in water, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P05019
For Use With (Application) Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
Formulation protein with no preservative
Gene ID (Entrez) 3479
Molecular Weight (g/mol) 7.5 kDa
Name Human IGF-I
Quantity 20 μg
Endotoxin Concentration <0.1 ng/ μg
Gene Alias C730016P09Rik; class 1 insulin-like growth factor I preproprotein; class 2 insulin-like growth factor I preproprotein; H-IGF-1; IBP1; IGF; IGF1; Igf-1; IGF1A; IGFI; Igf-I; IGFIa; IGF-IA; IGF-IB; insulin growth factor-1; Insulin like growth factor; insulin like growth factor 1; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor I (somatomedin C); insulin-like growth factor IB; mechano growth factor; MGF; M-IGF-1; OTTHUMP00000195084; prepro-IGF-I; prepro-insulin-like growth factor I; R-IGF-1; somatomedin; Somatomedin C; Somatomedin-C
Common Name IGF1
Biological Activity ED50 ≤ 2 ng/mL; determined by the dose-dependent proliferation of FDC-P1 cells.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Sequence GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
Content And Storage -20°C
Classification Carrier-Free
Activity 2 ng/ml
Bioassay proliferation of mouse FDC-P1 cells
Endotoxin Level <1 EU/ml
Shipping Condition Approved for shipment on Wet or Dry Ice
Expression System E. coli
Protein Family Growth Factors & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype IGFs (Insulin-Like Growth Factors)
Research Category Neurobiology, Drug Development, Oncology, Signal Transduction
Purity or Quality Grade ≥98% by SDS-PAGE and HPLC
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WARNING: Cancer - www.P65Warnings.ca.gov
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