Invitrogen IgG4 Human Uncoated ELISA Kit with Plates
Uncoated ELISA Kit with Plates
Manufacturer: Invitrogen 885059022
The Human IgG4 Uncoated ELISA Kit contains pre-matched antibody pair, platess and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IgG4. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Several studies have shown that a large population of patients with recurrent respiratory tract infection have low IgG4 concentrations.
|Colorimetric Microplate Reader|
|Plasma,10 μL|Serum,10 μL|Supernatant,10 μL|
|24 hr 30 min|
|10X Coating Buffer: Buffer for plating the Capture Antibody|
|2 x 96 tests|
|10μL (1:100 prediluted)|
|1 hr 45 min|
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