Invitrogen™ Human IL-18 ProQuantum Immunoassay Kit

ELISA

Supplier:  Invitrogen™ A35613

Catalog No. A35613

Description

Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.

• High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
• Broad dynamic range-≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
• Small sample consumption-use 2–5 μL of sample (compared to 75 μL for triplicate wells with other methods)
• Fast, easy workflow-2 hours from sample to answer, with no wash steps
• No proprietary instrument to purchase-runs on any real-time PCR instrument

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Specifications

• Accession Number: cytokine; iboctadekin; IFN-gamma-inducing factor; IGIF; il 18; IL-1 gamma; IL18; IL-18; IL1F4; IL-1 g; ILN; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; Interleukin18; Interleukin-18; interleukin-18 proprotein; interleukin-18-like protein; MGC12320
• Assay Sensitivity: <0.05 pg/mL
• Product Type: ProQuantum
• For Use With (Equipment): qPCR Instruments
• Gene Symbol: IL18
• Quantity: 96 Tests
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Target: IL-18
• Total Assay Time: 2 hrs

• Assay Range: 0.064 to 1000 pg/mL
• Conjugate: FAM™
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): 3606
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Test Time: 30 mins