Invitrogen™ Human IL-2 Instant ELISA™ Kit

Description

ELISA

The Human Interleukin 2 (Hu IL-2) ELISA quantitates Hu IL-2 in human serum, plasma, cell culture supernatants, amniotic fluid, synovial fluid, urine or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-2. Principle of the method The Human IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: P60568
• Assay Range: 18.8 to 1,200 pg/mL
• Assay Sensitivity: 2.3 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant, Synovial Fluid, Urine
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3558
• Gene Symbol: IL-2,TCGF,lymphokine
• Interassay CV: 7.4%
• Intraassay CV: 5.8
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Amniotic Fluid 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Synovial Fluid 50 μL, Urine 50 μL
• Storage Requirements: -20°C
• Synonym: Aldesleukin, T cell growth factor, TCGF
• Target: IL-2
• Test Time: 15 mins
• Total Assay Time: 3 hrs 10 mins