Invitrogen™ IL-2 Human Instant ELISA™ Kit

Instant ELISA Kit

Supplier:  Invitrogen™ BMS221INST

Catalog No. 50-180-50

Includes: Aluminum pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human IL-2, Biotin-Conjugate (anti-IL-2 polyclonal antibody), Sample Diluent and Streptavidin-HRP, lyophilized
Aluminum pouch(es) with a human IL-2 Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers

Description

The Human Interleukin 2 (Hu IL-2) ELISA quantitates Hu IL-2 in human serum, plasma, cell culture supernatants, amniotic fluid, synovial fluid, urine or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-2. Principle of the method The Human IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: P60568
• Assay Sensitivity: 2.3 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL-2,TCGF,lymphokine
• Intraassay CV: 5.8%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 18.8-1,200 pg/mL
• Conjugate: Biotin
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant, Synovial Fluid, Urine
• For Use With (Application): ELISA
• Gene ID (Entrez): 3558
• Interassay CV: 7.4%
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Quantity: 128 Tests
• Sample Volume: Amniotic Fluid, 50 μL; Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Synovial Fluid,50 μL; Urine,50 μL
• Synonym: Aldesleukin, T cell growth factor, TCGF
• Test Time: 20 min.

For Research Use Only