Invitrogen™ IL-2 Human ELISA Kit

The Human Interleukin-2 (Hu IL-2) ELISA quantitates Hu IL-2 in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-2.

Supplier:  Invitrogen™ BMS2212TWO

Catalog No. 50-112-5368

Description

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: P60568
• Assay Sensitivity: 9.1 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL-2,TCGF,lymphokine
• Intraassay CV: 7%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 30 min.

• Assay Range: 18.8-1,200 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 3558
• Interassay CV: 5%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Controls, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 2 x 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: Aldesleukin, T cell growth factor, TCGF
• Test Time: 1 hr. 20 min.

For Research Use Only