Gibco™ Human IL-23 Recombinant Protein, PeproTech®

Catalog No.	Quantity
20023100UG	100 μg
200-23-10UG	10 μg
20-023-01M	2 x 500 μg
20023250UG	250 μg
200-23-2UG	2 μg
20023500UG	500 μg
200-23-50UG	50 μg

Catalog No. 20023100UG Supplier Gibco™ Supplier No. 20023100UG

Description

Recombinant Protein

200-23-1MG will be provided as 2 x 500 μg (200-23-500UG). Recombinant Human IL-23 is a 53.5 kDa, heterodimeric protein consisting of the two subunits, p19 (170 amino acids) and p40 (306 amino acids). This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 that is disulfide-linked with to two subunits, a p19 subunit unique to IL-23, and a p40 subunit that is shared with IL-12. IL-23 interacts with IL-12Rbeta1 and a beta2-like receptor subunit with STAT4 binding domain, IL-23R. IL-23 is secreted by activated mouse and human dendritic cells and is involved in the inflammation process and associated with autoimmune diseases. Mouse IL-23 was found not to induce significant amounts of IFN-gamma, induces strong proliferation of memory T cells (but not naive T cells), and activate mouse memory T cells to produce the proinflammatory cytokine IL-17. On the other hand, human IL-23 induces proliferation of memory T cells, induces moderate levels of IFN-gamma, and is produced by naive and memory T cells. IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance, and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines. IL-23 interacts with the receptors IL-12R beta1 and IL-23R to activate the Jak-Stat signaling cascade. IL-23 has been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.

Specifications

• Accession Number: Q9NPF7
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 51561
• Molecular Weight (g/mol): 53.5 kDa
• Name: Human IL-23
• Quantity: 100 μg
• Source: Insect cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: IL23; IL-23; IL-23 subunit alpha; IL23A; IL-23 A; IL-23-A; IL23P19; IL-23p19; ILN; Interleukin; interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; P19; SGRF; UNQ2498/PRO5798
• Common Name: IL-23
• Gene Symbol: IL23A
• Biological Activity: Measured by its ability to induce IL-17 secretion by mouse splenocytes.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: p19 subunit: RAVPGGSSPA WTQCQQLSQK LCTLAWSAHP LVGHMDLREE GDEETTNDVP HIQCGDGCDP QGLRDNSQFC LQRIHQGLIF YEKLLGSDIF TGEPSLLPDS PVGQLHASLL GLSQLLQPEG HHWETQQIPS LSPSQPWQRL LLRFKILRSL QAFVAVAARV FAHGAATLSP p40 subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
• Content And Storage: -20°C
• Expression System: Insect cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.