Invitrogen™ Human IL-7 R alpha/CD127 ELISA Kit

Description

ELISA

Human IL-7 R alpha/CD127 quantitates human IL-7 R alpha/CD127 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human IL-7 R alpha/CD127.

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

Specifications

• Accession Number: CD127; CD127 antigen; CDW127; IL 7 receptor; IL 7 R a; IL 7 R subunit alpha; IL 7 R a; IL 7 Ra; IL 7 Ra; IL7 receptor; IL-7 receptor alpha chain; IL7 receptor subunit alpha; IL-7 receptor subunit alpha; Il7r; IL-7 R; IL7R a; IL7R alpha; IL7R subunit alpha; IL-7 R subunit alpha; IL7R a; IL7RA; IL-7 RA; IL-7 Ralpha; IL-7 R-alpha; IL7Ra; ILRA; interleukin 7 receptor; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin7 receptor subunit alpha; interleukin-7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7 R
• Assay Range: 0.14 to 100 ng/mL
• Assay Sensitivity: 0.14 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3575
• Gene Symbol: Il7r
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CD127
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins