Invitrogen™ Human IL-2 Recombinant Protein

Description

Recombinant Protein

Mature human IL-2 was expressed in Pichia pastoris, X-33. GenBank Accession # NP_000577, amino acids 21 - 153. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: APTSSSTKKT. Purity is >95% by SDS-PAGE analyses. Activity: The biological activity was determined by measuring the proliferation of mouse HEK-Blue cells. Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments. Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 1 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: P60568
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: PBS with no preservative
• Gene ID (Entrez): 3558
• Molecular Weight (g/mol): 15 kDa
• Name: Human IL-2
• Quantity: 20 μg
• Source: Yeast
• Regulatory Status: RUO
• Gene Alias: aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
• Common Name: IL-2
• Gene Symbol: IL2
• Biological Activity: ED50 = 13.5- 20.3 ng/mL; determined by measuring the proliferation of mouse HEK-Blue cells.
• Conjugate: Unconjugated
• Species: Yeast
• Recombinant: Recombinant
• Sequence: Mouse IL-2, amino acids 21-153 (Accession # NP_000577)
• Content And Storage: 4°C
• Expression System: P. pastoris
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95% by SDS-PAGE