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  5. Invitrogen™ Human JAM-C ELISA Kit 

Invitrogen™ Human JAM-C ELISA Kit

Catalog No. EH285RBX10
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Catalog No. EH285RBX10 Supplier Invitrogen™ Supplier No. EH285RBX10
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Description

ELISA

Human JAM-C quantitates human JAM-C in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human JAM-C.

JAM3 is involved in tight junctions and represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number 1110002N23Rik; JAM-2; JAM3; JAM-3; JAMC; JAM-C; Jcam3; junction adhesion molecule 3; junction cell adhesion molecule 3; junction cell adhesion molecule C; junctional adhesion molecule 3; Junctional adhesion molecule C; sJAM-C; Soluble form of JAM-C; UNQ859/PRO1868
Assay Range 0.12 to 30 ng/mL
Assay Sensitivity 0.12 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 83700
Gene Symbol JAM3
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target JAM3
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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