Invitrogen™ Human Kallikrein 1 His-tag Recombinant Protein

Catalog No.	Quantity
PIRP88085	50 μg
PIRP88086	1 mg

Catalog No. PIRP88085 Supplier Invitrogen™ Supplier No. RP88085

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (1205 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.

Specifications

• Accession Number: P06870
• For Use With (Application): Control, Bioactivity
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 3816
• Molecular Weight (g/mol): 50 kDa
• Name: Human Kallikrein 1 His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 50 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: 0610007D04Rik; 7S nerve growth factor gamma chain; 7S nerve growth factor gamma subunit; esterase B; Gamma-NGF; glandular kallikrein 1; glandular kallikrein K1; Glandular kallikrein K3; glandular kallikrein-7, submandibular/renal; hK1; Kal; KALA; KAL-B; kallikrein 1; Kallikrein 1 renal/pancreas/salivary; kallikrein 1, renal/pancreas/salivary; kallikrein 1-like peptidase; kallikrein 1-related peptidase b3; kallikrein 1-related peptidase C7; kallikrein 5; kallikrein 6; kallikrein 7; kallikrein renal/pancreas/salivary; kallikrein serine protease 1; kallikrein-1; Kallikrein-related protein K1; kidney/pancreas/salivary gland kallikrein; Klk1; Klk-1; Klk1b3; Klk1b6; Klk1c1; Klk1c7; Klk1l; Klk3; Klk-3; Klk5; Klk6; Klk-6; Klk7; Klk-7; KLKR; mGk-3; mGK-6; Nerve growth factor gamma chain; Nerve growth factor gamma chain 1; Nerve growth factor gamma chain 2; nerve growth factor gamma polypeptide; nerve growth factor, gamma; Ngfg; Pancreatic kallikrein; proteinase A; PS kallikrein; RATKALA; Renal kallikrein; RGK-1; RGK-7; rK-1; RSKG-7; Tissue kallikrein; tissue kallikrein-3; Tissue kallikrein-6; True kallikrein; True tissue kallikrein
• Common Name: Kallikrein 1
• Gene Symbol: KLK1
• Biological Activity: Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >1,500 pmoles/min/ μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human KLK1 precursor, amino acids Met1-Ser262 (Accession # NP_002248.1) with a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >1,500 pmoles/min/ μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.