Gibco™ Human KLF4-TAT Recombinant Protein, PeproTech®

Catalog No.	Quantity
110-08-5UG	5 μg
11008100UG	100 μg
11-008-500	5 x 100 μg
110-08-25UG	25 μg

Catalog No. 110-08-5UG Supplier Gibco™ Supplier No. 110085UG

Description

Recombinant Protein

110-08-1MG will be provided as 10 x 100 μg (110-08-100UG). 110-08-250UG will be provided as multiple 25 μg (110-08-25UG) and 100 μg (110-08-100UG) vials. 110-08-500UG will be provided as 5 x 100 μg (110-08-100UG). Recombinant Human KLF4-TAT is a 483 amino acid protein, including a 13-residue C-terminal TAT peptide, with a calculated molecular weight of 51.7 kDa. PeproTech's Recombinant Human KLF4-TAT is a mixture of the expected sequence beginning at Met1 and a truncated isoform beginning at Tyr54. Due to post-translational modifications, SDS-PAGE gel shows bands at approximately 72 and 66kDa, under reduced conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

KLF4 is a member of the Kruppel-like factor (KLF) family of zinc finger transcription factors. Members of this family share 3 contiguous C2H2-type zinc fingers at the carboxyl terminus that comprise the DNA-binding domain. KLF4 is highly expressed in skin and gut epithelial tissues, but is also found in various other cells and tissues, including vascular endothelial cells, lymphocytes, lung, and testis. It is an important regulator of the cell cycle, transcription, and cell differentiation. Together with Sox2, Oct4, and cMyc, KLF4 can induce the reprogramming of primary human fibroblasts to a pluripotent state. KLF4 and other transcription factors can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary, as well as transformed, cells.

Specifications

• Accession Number: O43474
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 9314
• Molecular Weight (g/mol): 51.7 kDa
• Name: Human KLF4-TAT
• Quantity: 5 μg
• Source: HEK293 cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: KLF4
• Common Name: KLF4-TAT
• Gene Symbol: KLF4
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: TAT-fusion
• Sequence: MAVSDALLPS FSTFASGPAG REKTLRQAGA PNNRWREELS HMKRLPPVLP GRPYDLAAAT VATDLESGGA GAACGGSNLA PLPRRETEEF NDLLDLDFIL SNSLTHPPES VAATVSSSAS ASSSSSPSSS GPASAPSTCS FTYPIRAGND PGVAPGGTGG GLLYGRESAP PPTAPFNLAD INDVSPSGGF VAELLRPELD PVYIPPQQPQ PPGGGLMGKF VLKASLSAPG SEYGSPSVIS VSKGSPDGSH PVVVAPYNGG PPRTCPKIKQ EAVSSCTHLG AGPPLSNGHR PAAHDFPLGR QLPSRTTPTL GLEEVLSSRD CHPALPLPPG FHPHPGPNYP SFLPDQMQPQ VPPLHYQELM PPGSCMPEEP KPKRGRRSWP RKRTATHTCD YAGCGKTYTK SSHLKAHLRT HTGEKPYHCD WDGCGWKFAR SDELTRHYRK HTGHRPFQCQ KCDRAFSRSD HLALHMKRHF GGYGRKKRRQ RRR
• Content And Storage: -20°C
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 90% by SDS-PAGE gel and HPLC analyses.