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Gibco™ Human LIF Recombinant Protein

Catalog No. PHC9484
Encompass_Preferred
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Quantity:
10 μg
100 μg
1000 μg

Recombinant Protein

Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water or appropriate buffered solution containing 0.1% BSA to regain full activity. Apportion there constituted protein into working aliquots and store at -20°C. Make any further dilutions of the reconstituted protein in buffered solution containing a carrier protein, such as PBS with 0.1% BSA. Storage: Store the lyophilized protein at 2-8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P15018
For Use With (Application) Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
Formulation protein with no preservative
Gene ID (Entrez) 3976
Molecular Weight (g/mol) 21 kDa
Name Human LIF
Purification Method Sequential Chromatography
Quantity 10 μg
Endotoxin Concentration <0.1 ng/μg
Gene Alias CDF; cholinergic differentiation factor; cholinergic neuronal differentiation factor; d factor; DIA; differentiation inhibitory activity; differentiation-inducing factor; differentiation-stimulating factor; Emfilermin; hepatocyte-stimulating factor III; HILDA; human interleukin in DA cells; leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); LIF; Melanoma-derived LPL inhibitor; MLPLI; myeloid leukaemia inhibitory factor
Common Name LIF
Gene Symbol LIF
Biological Activity ED50 = 0.05 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Sequence PLPITPVNAT CAIRHPCHNN LMNQIRSQLA QLNGSANALF ILYYTAQGEP FPNNLDKLCG PNVTDFPPFH ANGTEKAKLV ELYRIVVYLG TSLGNITRDQ KILNPSALSL HSKLNATADI LRGLLSNVLC RLCSKYHVGH VDVTYGPDTS GKDVFQKKKL GCQLLGKYKQ IIAVLAQAF
Content And Storage 4°C
Activity 0.5 ng/ml
Bioassay Dose-dependent proliferation of TF-1 cells
Shipping Condition Dry Ice
Expression System E. coli
Protein Family Cytokines & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype Other Cytokines
Research Category Oncology
Product Line Gibco™
Purity or Quality Grade ≥95% by SDS-PAGE
Protein Leukemia inhibitory factor
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WARNING: Cancer - www.P65Warnings.ca.gov
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