Gibco™ Human LIGHT Recombinant Protein, PeproTech®

Catalog No.	Quantity
31009B15UG	15 μg
310-09B-3UG	3 μg

Catalog No. 31009B15UG Supplier Gibco™ Supplier No. 31009B15UG

Description

Recombinant Protein

310-09B-100UG will be provided as 15 μg (310-09B-15UG) vials. 310-09B-1MG will be provided as 15 μg (310-09B-15UG) vials. 310-09B-250UG will be provided as 15 μg (310-09B-15UG) vials. 310-09B-500UG will be provided as 15 μg (310-09B-15UG) vials. Recombinant Human LIGHT has a calculated mass of 19.3 kDa, containing 177 amino acid residues. Due to glycosylation, LIGHT migrates between 20.0-22.5 kDa by SDS-PAGE under non-reducing conditions. *Human LIGHT(Catalog Number 310-09B) has replaced Human LIGHT(Catalog Number 310-09) This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.

Specifications

• Accession Number: O43557
• For Use With (Application): Control, Western Blot Control, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 8740
• Molecular Weight (g/mol): 19.3 kDa
• Name: Human LIGHT
• Purification Method: Purified
• Quantity: 15 μg
• Source: Insect cells
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: CD258; Herpes virus entry mediator ligand; herpesvirus entry mediator ligand; HVEML; HVEM-L; Light; LTg; Ly113; sCD258; sLIGHT; soluble CD258; soluble LIGHT; Tnfsf14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand 1D; tumor necrosis factor ligand superfamily member 14; Tumor necrosis factor ligand superfamily member 14, membrane form; Tumor necrosis factor ligand superfamily member 14, soluble form; tumor necrosis factor superfamily member 14; UNQ391/PRO726
• Common Name: LIGHT
• Gene Symbol: TNFSF14
• Biological Activity: Determined by its ability to induce IL-8 secretion in human BEAS-2 B cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: RLGEMVTRLP DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV
• Content And Storage: -20°C
• Activity: Determined by its ability to induce IL-8 secretion in human BEAS-2B cells.
• Expression System: Insect cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.