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  5. Invitrogen™ Human Lp-PLA2/PLA2G7/PAF-AH ELISA Kit 

Invitrogen™ Human Lp-PLA2/PLA2G7/PAF-AH ELISA Kit

Catalog No. EH304RB
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EH304RB 96 Tests
EH304RBX5 5 x 96 Tests
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Description

ELISA

Human Lp-PLA2/PLA2G7/PAF-AH quantitates human Lp-PLA2/PLA2G7/PAF-AH in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Lp-PLA2/PLA2G7/PAF-AH.

Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of thesn2 ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL-associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); LDL-PLA2; lipoprotein-associated phospholipase A2; LP-PLA2; PAF 2-acylhydrolase; PAF acetylhydrolase; PAFAD; PAFAH; phospholipase A2 group VII; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); PLA2G7; Platelet-activating factor acetylhydrolase; R75400
Assay Range 8.19 to 2,000 ng/mL
Assay Sensitivity 8.1 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 7941
Gene Symbol PLA2G7
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target PLA2G7
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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