Invitrogen™ Human M-CSF Recombinant Protein

Description

Recombinant Protein

Macrophage-CSF (human) Recombinant Protein, RP-8643, from Invitrogen.

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells. Recombinant human M-CSF is a disulfide linked homodimer, containing two 159 amino acid chains, with a total molecular weight of 36.8 kDa. Purity is typically greater than 97% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent proliferation of mouse NFS-60 cells and is typcially less than 2.0ng/mL. The specific activity of this protein is 5x10^5 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability. Amino acid sequence: MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS.

Specifications

• Accession Number: P09603
• Concentration: 0.1mg/mL
• For Use With (Application): Control
• Formulation: PBS with no preservative; pH 8.0
• Gene ID (Entrez): 1435
• Molecular Weight (g/mol): ∽36.85
• Name: Human M-CSF
• pH Range: 8.0
• Quantity: 10 μg
• Source: E. coli
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: H-MCSF
• Common Name: Human M-CSF
• Gene Symbol: CSF1
• Specific Reactivity: Human
• Conjugate: Unconjugated
• Cross Reactivity: Hu
• Species: E. coli
• Recombinant: Recombinant
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: purified

in vitro research only