Abnova™ Human MAP2K1 (NP_002746.1, 1 a.a. - 393 a.a.) P124L mutant Full length Protein with GST-tag co-expressed with His-tagged RAF1 (NP_002871.1, 306 a.a. - 648 a.a.) Y340D/Y341D mutant Full length Recombinant Protein in Baculovirus infected Sf21 cells

Used for Func, SDS-PAGE

Manufacturer:  Abnova™ P5746

Catalog No. 89-951-573

Description

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq]

Specifications

• Accession Number: NP_002746.1
• Format: Liquid
• Gene ID (Entrez): 5604
• Name: MAP2K1 (P124L) (Human) Recombinant Protein
• Purification Method: Ni-NTA affinity chromatography
• Quality Control Testing: SDS-PAGE Stained with Coomassie Blue
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Gene Alias: MAPKK1/MEK1/MKK1/PRKMK1
• Gene Symbol: MAP2K1
• Host Species: Insect
• Protein Tag: GST

• For Use With (Application): Functional Study, Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis
• Formulation: Liquid
• Molecular Weight: 70kDa
• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Purity: 97% by SDS-PAGE/CBB staining
• Quantity: 5 ug
• Regulatory Status: RUO
• Common Name: MAP2K1
• Biological Activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
  Substrate : Erk2 inactive mutant. ATP: 100 µM.
• Recombinant: Yes
• Expression System: Baculovirus infected insect cell (Sf21) expression system