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Abnova™ Human MAP3K14 (NP_003945.1, 658 a.a. - 1114 a.a.) Partial Recombinant Protein with GST-tag expressed in Baculovirus infected Sf21 cells

Catalog No. 89951632 Shop All Abnova Corporation Products
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89-951-632 5 μg
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Catalog No. 89-951-632 Supplier Abnova™ Supplier No. P5725
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Used for Func, SDS-PAGE

This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq]

Specifications

Accession Number NP_003945.1
For Use With (Application) Functional Study, SDS-PAGE
Formulation Liquid
Gene ID (Entrez) 9020
Molecular Weight (g/mol) 96kDa
Name MAP3K14 (Human) Recombinant Protein
Preparation Method Baculovirus infected insect cell (Sf21) expression system
Purification Method Glutathione sepharose chromatography
Quality Control Testing Loading 1 ug protein in SDS-PAGE
Quantity 5 μg
Storage Requirements Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Regulatory Status RUO
Gene Alias FTDCR1B/HS/HSNIK/NIK
Common Name MAP3K14
Gene Symbol MAP3K14
Biological Activity The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
Substrate : BTN-IKKa [inactive mutant]. ATP: 100 μM.
Species Baculovirus
Recombinant Recombinant
Protein Tag GST
Expression System Baculovirus infected insect cell (Sf21) expression system
Form Liquid
Purity or Quality Grade 76% by SDS-PAGE/CBB staining.
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