Gibco™ Human MIA Recombinant Protein, PeproTech®

Catalog No.	Quantity
13001250UG	250 μg
13001100UG	100 μg
130-01-1MG	1 mg
130-01-20UG	20 μg
13001500UG	500 μg
130-01-5UG	5 μg

Catalog No. 13001250UG Supplier Gibco™ Supplier No. 13001250UG

Description

Recombinant Protein

Recombinant Human MIA is a 12.2 kDa globular protein containing 108 amino acid residues, including two intramolecular disulfide bonds. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Tumorigenesis is a process that is mediated by a network of membrane, cytosolic and nuclear associated factors, which regulate proliferation and cell-matrix interaction through signaling cascades. The phenotype of malignant melanomas in vivo depends on the global expression of stimulatory or inhibitory factors generated in both the tumors cells and their environment. One example, Melanoma inhibitory activity (cartilage-derived retinoic acid-sensitive protein (CD-RAP), MIA) is a Src homology 3 (SH3)-like domain containing protein that is secreted from chondrocytes and malignant melanoma cells. MIA is translated as a 131-amino acid pro-form and processed into a mature 107-amino acid protein after cleavage of a secretion signal. MIA is expressed during chondrogenesis and in mature chondrocytes, suggesting that MIA is necessary for normal cartilage cell phenotype. MIA mRNA is present in carcinomas of the colon, ovary, kidney, and head/neck, and may represent a marker to monitor melanomic activity.

Specifications

• Accession Number: Q16674
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 8190
• Molecular Weight (g/mol): 12.2 kDa
• Name: Human MIA
• Quantity: 250 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: cartilage derived retinoic acid sensitive protein; cartilage-derived retinoic acid-sensitive protein; Cdrap; CD-RAP; melanoma inhibitory activity; melanoma inhibitory activity 1; melanoma inhibitory activity protein; melanoma inhibitory activity/condrocyte-derived retinoic acid sensitive protein homolog (MIA/CD-RAP); melanoma-derived growth regulatory protein; melanoma-inhibitory-activity; MIA; Mia1
• Common Name: MIA
• Gene Symbol: MIA
• Biological Activity: The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 ug/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.