Gibco™ Human MMP-1 Recombinant Protein, PeproTech®

Description

Recombinant Protein

420-01-1MG will be provided as 4 x 250 μg (420-01-250UG) 420-01-500UG will be provided as 2 x 250 μg (420-01-250UG) Recombinant Human MMP-1 is a 42.7 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain, which is involved in substrate specificity, and in binding TIMP-1. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.

Specifications

• Accession Number: P03956
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 4312
• Molecular Weight (g/mol): 42.7 kDa
• Name: Human MMP-1
• Quantity: 10 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: 22 kDa interstitial collagenase; 27 kDa interstitial collagenase; CLG; CLGN; Collagenase; collagenase-like A; fibroblast collagenase; Interstitial collagenase; interstitial collagenase A; matrix metallo protease; matrix metallopeptidase 1; matrix metallopeptidase 1 (interstitial collagenase); matrix metallopeptidase 1 A; matrix metallopeptidase 1 A (interstitial collagenase); matrix metalloprotease 1; matrix metalloproteinase 1; matrix metalloproteinase 1 A (interstitial collagenase); Matrix metalloproteinase1; Matrix metalloproteinase-1; Matrix metalloproteinase-1 A; McolA; Mcol-A; MMP; MMP1; MMP-1; Mmp1a; MMP-1 A; MMPs
• Common Name: MMP1
• Gene Symbol: MMP1
• Biological Activity: MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 ug/ml, 50% cleavage was achieved (at an incubation time of approximately 25 minutes.)
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEKAF QLWSNVTPLT FTKVSEGQAD IMISFVRGDH RDNSPFDGPG GNLAHAFQPG PGIGGDAHFD EDERWTNNFR EYNLHRVAAH ELGHSLGLSH STDIGALMYP SYTFSGDVQL AQDDIDGIQA IYGRSQNPVQ PIGPQTPKAC DSKLTFDAIT TIRGEVMFFK DRFYMRTNPF YPEVELNFIS VFWPQLPNGL EAAYEFADRD EVRFFKGNKY WAVQGQNVLH GYPKDIYSSF GFPRTVKHID AALSEENTGK TYFFVANKYW RYDEYKRSMD PGYPKMIAHD FPGIGHKVDA VFMKDGFFYF FHGTRQYKFD PKTKRILTLQ KANSWFNCRK N
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 90% by SDS-PAGE gel and HPLC analyses.