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Human/Mouse Active Caspase-3 Antibody, R&D Systems™
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Catalog No. AF835
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50 μg
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AF835 50 μg
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Description

Rabbit Polyclonal Antibody has been used in 155 publications

Caspase-3 Polyclonal specifically detects Caspase-3 in Human, Mouse samples. It is validated for Immunohistochemistry, Immunocytochemistry.

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Specifications

Antigen Caspase-3
Applications Immunohistochemistry, Immunocytochemistry
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Immunohistochemistry 1-15 ug/mL, Immunocytochemistry 5-15 ug/mL
Gene Accession No. P42574
Gene Alias Apopain, apoptosis-related cysteine protease, CASP3, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, Caspase3, CPP32, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, LICE-1, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
Gene Symbols CASP3
Host Species Rabbit
Immunogen KLH-coupled human Caspase-3 synthetic peptide CRGTELDCGIETD Accession # P42574
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 836
Test Specificity Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R&D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Human, Mouse
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype IgG
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