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Human/Mouse Cleaved Caspase-3 (Asp175) Antibody, R&D Systems™
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Catalog No. MAB835
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50 μg
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MAB835 50 μg
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Description

Rabbit Monoclonal Antibody has been used in 52 publications

Caspase-3 Monoclonal specifically detects Caspase-3 in Human, Mouse samples. It is validated for Western Blot, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Immunocytochemistry.

Specifications

Antigen Caspase-3
Applications Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry
Classification Monoclonal
Clone 269518
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 0.5 ug/mL, Immunohistochemistry 0.3-25 ug/mL, Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. U26943
Gene Alias Apopain, apoptosis-related cysteine protease, CASP3, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, Caspase3, CPP32, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, LICE-1, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
Gene Symbols CASP3
Host Species Rabbit
Immunogen KLH-conjugated human Caspase-3 synthetic peptide CRGTELDCGIETD Accession # U26943
Purification Method Protein A or G purified from hybridoma culture supernatant
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 836
Test Specificity Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Human, Mouse
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Purified
Isotype IgG
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