Human/Mouse/Rat alpha-Smooth Muscle Actin Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 59 publications

Supplier:  R&D Systems MAB1420

Catalog No. MAB1420

Description

alpha-Smooth Muscle Actin Monoclonal specifically detects alpha-Smooth Muscle Actin in Human, Mouse, Rat samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready, Dual RNAscope ISH-IHC.

Specifications

• Antigen: alpha-Smooth Muscle Actin
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Western Blot 0.1-0.25 ug/mL, Simple Western 10 ug/mL, Immunohistochemistry 8-25 ug/mL, Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL, CyTOF-ready, Dual RNAscope ISH-IHC 0.5-25 ug/mL
• Gene Alias: AAT6, ACTA2, Actin alpha 2, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, ACTSA, ACTVS, alpha 2 actin, alpha SMA, alpha smooth muscle actin, alpha-actin-2, alpha-SMA, alphaSmooth Muscle Actin, a-sma, Cell growth-inhibiting gene 46 protein, growth-inhibiting gene 46, MYMY5, sma, smooth muscle actin
• Host Species: Mouse
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Gene ID (Entrez): 59
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG2a

• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry, CyTOF, In Situ Hybridization (ISH)
• Clone: 1A4
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Symbols: ACTA2
• Immunogen: Peptide corresponding to residues EEEDSTALVC of the N-term of human alpha-smooth muscle actin (1)
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human alpha-Smooth Muscle Actin.
• Target Species: Human, Mouse, Rat
• Form: Purified