Gibco™ Human/Mouse/Rat BDNF Recombinant Protein, PeproTech®

Catalog No.	Quantity
45002250UG	250 μg
45002100UG	100 μg
450-02-10UG	10 μg
450-02-1MG	1 mg
450-02-2UG	2 μg
45002500UG	500 μg
450-02-50UG	50 μg

Catalog No. 45002250UG Supplier Gibco™ Supplier No. 45002250UG

Description

Recombinant Protein

Recombinant Human/Murine/Rat BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

Specifications

• Accession Number: P21237, P23363, P23560
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 12064, 24225, 627
• Molecular Weight (g/mol): 27 kDa
• Name: Human/Mouse/Rat BDNF
• Quantity: 250 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: abrineurin; ANON2; anorexia BDNF; Bdnf; BDNF precursor form; bdnf protein; brain derived neurothrophic factor; brain derived neurotrophic factor; brain-derived neurotrophic factor; BULN2; H-BDNF; neurotrophin; ProBDNF
• Common Name: BDNF
• Gene Symbol: BDNF
• Biological Activity: Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.