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Human/Mouse TNF-alpha Antibody, R&D Systems™
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Catalog No. AF410SP
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25 μg
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AF410SP 25 μg
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Goat Polyclonal Antibody has been used in 74 publications

TNF-alpha Polyclonal specifically detects TNF-alpha in Human, Mouse samples. It is validated for Western Blot, Immunohistochemistry, Neutralization, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready, ELISA Capture (Matched Antibody Pair).

Specifications

Antigen TNF-alpha
Applications Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, CyTOF
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 0.1 ug/mL, Neutralization 0.1-0.4 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, Immunocytochemistry 5-15 ug/mL, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. P06804
Gene Alias APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Gene Symbols TNF
Host Species Goat
Immunogen E. coli-derived recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) Leu80-Leu235 Accession # P06804
Purification Method Affinity Purified
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 7124
Test Specificity Detects mouse TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF-alpha is observed and less than 1% cross-reactivity with recombinant human TNF-alpha is observed.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Mouse
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype IgG
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