Invitrogen™ Human Myeloperoxidase Instant ELISA™ Kit

Description

ELISA

The Human Myeloperoxidase (Hu MPO) ELISA quantitates Hu MPO in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MPO. Principle of the method The Human MPO solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Myeloperoxidase (MPO) is a heme protein that is synthesized during myeloid differentiation and is a major component of neutrophil azurophilic granules. It is produced as a single chain precursor and subsequently cleaved into a light and heavy chain. The mature MPO is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme is responsible for producing hypohalous acids that are central to the microbicidal activity of neutrophils. Diseases that are associated with MPO include Myeloperoxidase Deficiency and Alzheimer's Disease, Familial, 1.

Specifications

• Accession Number: P05164
• Assay Range: 156 to 10,000 pg/mL
• Assay Sensitivity: 26 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4353
• Gene Symbol: MPO
• Interassay CV: 9.5%
• Intraassay CV: 6.2
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Storage Requirements: -20°C
• Synonym: Myeloperoxidase
• Target: Myeloperoxidase
• Test Time: 15 mins
• Total Assay Time: 3 hrs 10 mins

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov