Gibco™ Human MRP4 Vesicles

Catalog No.	Quantity
GM0012	0.5 mL

Catalog No. GM0012 Supplier Gibco™ Supplier No. GM0012

Description

Human MRP4 Vesicles

Human MRP4 Vesicles are insect derived purified plasma membranes with inserted MRP4 transport protein (multidrug resistance-associated protein 4, ABCC4). Our Human MRP4 Vesicles can be used to evaluate test compounds and drug interactions with MRP4 in in vitro assays. MRP4 is an ATP-binding cassette (ABC) transporter that carries organic anions, including nucleic acid derivatives, prostaglandins, and methotrexate.

Use Human MRP4 Vesicles to:

Investigate the transporter interactions of your drug candidates

Assess potential for transporter-mediated drug-drug interactions

Obtain high quality results with a large signal to noise ratios

Concept Behind ABC Transporter Vesicles
ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate's substrate and drug interaction potential. While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles.

Clear and Reliable Results
Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these 'inside-out' vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter.

Transporter Vesicle Details
Human MRP4 Vesicles are part of a larger group of transporter vesicles known as ABC transporter vesicles. They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing MRP4. MRP4 is highly expressed in proximal uriniferous tubules in the kidney, and plays an important role in drug excretion from the kidney. MRP4 has also been identified in vascular endothelial cells of the brain, where it greatly influences drug behavior at the blood-brain barrier.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Specifications

• Host Species: Human
• Grade: Research Grade
• For Use With (Application): Pharma and Biopharma
• Packaging: Vial
• Content And Storage: -80°C
• Shipping Condition: Dry Ice
• Source: Insect derived purified plasma membranes with inserted MRP4 transport protein (multidrug resistance-associated protein 4, ABCC4)
• Quantity: 0.5 mL
• Product Type: Human MRP4 Vesicles
• Form: Frozen

Frequently Asked Questions (FAQs)

What is the supplied quantity of ATP-binding cassette (ABC) Transporter Vesicles?

The quantity supplied is 2.5 mg total protein/500 µL per vial. The protein concentration is 5 mg/mL. When our recommended protocol is followed, 50 vesicular transport assays or 125 ATPase assays can be performed with one vial of ABC Transporter Vesicles product.

What is the storage condition for ATP-binding cassette (ABC) Transporter Vesicles?

We recommend storing them at -80 degrees C.

What are ATP-binding cassette (ABC) Transporter Vesicles?

Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, ATP-binding cassette (ABC) Transporter Vesicles are “inside-out” vesicles designed for use in vesicular transport assays. They allow the investigation of possible interactions between specific ABC transporters and test compounds. ABC Transporter Vesicles provide high levels of transporter activity with low background, giving a clear signal if the test compound is a substrate or inhibitor of a specific efflux transporter.

While ABC transporters typically mediate the efflux of substrates from cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for the test compound by determining the amount incorporated into the vesicles.
Note: One vial of vesicle product can perform 50 vesicular transport assays.

For Research Use Only. Not for use in diagnostic procedures.