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Gibco™ Human Nanog-TAT Recombinant Protein, PeproTech®

Numéro de catalogue. 12021B100UG
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Numéro de catalogue. Quantity
12021B100UG 100 μg
120-21B-1MG 1 mg
12021B250UG 250 μg
12021B500UG 500 μg
120-21B-5UG 5 μg
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Numéro de catalogue. 12021B100UG Fournisseur Gibco™ Code fournisseur 12021B100UG
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Description

Recombinant Protein

Recombinant Human Nanog-TAT is a 36.1 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13- residue C-terminal TAT peptide. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Nanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, that helps maintain pluripotency and self renewal via a STAT3-independent pathway. The introduction of nanog, along with Sox2, Oct4, and Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary, as well as transformed, cells.
TRUSTED_SUSTAINABILITY

Spécifications

Accession Number Q9H9S0
For Use With (Application) Functional Assay
Formulation protein with no preservative
Gene ID (Entrez) 79923
Molecular Weight (g/mol) 36.1 kDa
Name Human Nanog-TAT
Quantity 100 μg
Source E. coli
Regulatory Status RUO
Endotoxin Concentration <1 EU/ μg
Gene Alias Nanog
Common Name Nanog-TAT
Gene Symbol NANOG
Conjugate Unconjugated
Recombinant Recombinant
Protein Tag TAT-fusion
Sequence SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDVGGYGRK KRRQRRR
Content And Storage -20°C
Expression System E. coli
Form Lyophilized
Purity or Quality Grade ≥ 95% by SDS-PAGE gel and HPLC analyses.
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