Invitrogen™ Human Nectin-2 (CD112) Fc Chimera Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (1493 μL) be added to the vial to prepare a stock solution of 0.67 mg/mL. Concentration is measured by UV.

This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Specifications

• Accession Number: Q92692
• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: 50 mM tris with <0.1 μg/mL BSA, 0.01% mannitol, 0.01% Tween 80, 100 mM glycine, 10 mM NaCl, 5-8% trehalose and no preservative; pH 7.5
• Gene ID (Entrez): 5819
• Molecular Weight (g/mol): 70-80 kDa
• Name: Human Nectin-2 (CD112) Fc Chimera
• Purification Method: SDS-PAGE
• Quantity: 100 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: AI325026; AI987993; CD112; herpes virus entry mediator B; Herpesvirus entry mediator B; herpesvirus entry protein B; hveB; mHveB; MPH; Murine herpes virus entry protein B; murine herpesvirus entry protein B; nectin cell adhesion molecule 2; Nectin2; Nectin-2; Poliovirus receptor homolog; poliovirus receptor related 2; poliovirus receptor-like 2; poliovirus receptor-related 2; poliovirus receptor-related 2 (herpesvirus entry mediator B); poliovirus receptor-related protein 2; poliovirus sensitivity; PRR2; Pvr; Pvrl2; PVRR2; Pvs
• Common Name: Nectin 2
• Gene Symbol: NECTIN2
• Biological Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human CD226 at 2 μg/ml (100 μL/well) can bind human CD112 with a linear range of 6.4-800 ng/mL 2. Immobilized PVRIG Protein, Cynomolgus, Rhesus, Recombinant (His Tag) (at 2 μg/ml (100 μL/well) can bind Nectin-2 Protein, Human, Recombinant (hFc Tag), the EC50 of Nectin-2 Protein, Human, Recombinant (hFc Tag)is 0.2-1.2 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human CD112, amino acids Met1-Leu360 (Accession # NP_002847.1) with a C-terminal human IgG1 Fc region
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human CD226 (at 2 μg/ml (100 μL/well) can bind human CD112 with a linear range of 6.4-800 ng/mL 2. Immobilized PVRIG Protein, Cynomolgus, Rhesus, Recombinant (His Tag) (at 2 μg/ml (100 μL/well) can bind Nectin-2 Protein, Human, Recombinant (hFc Tag), the EC50 of Nectin-2 Protein, Human, Recombinant (hFc Tag)is 0.2-1.2 ng/mL))
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.