Invitrogen™ Human Notch-3 ELISA Kit

Description

ELISA

Human Notch-3 quantitates human Notch-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Notch-3.

NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction (with its cell-bound ligands delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human. NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

Specifications

• Accession Number: AW229011; CADASIL; CADASIL1; CASIL; hpbk; IMF2; LMNS; N3; neurogenic locus notch homolog protein 3; Notch 3; Notch 3 extracellular truncation; Notch 3 intracellular domain; Notch gene homolog 3; Notch homolog 3; Notch3
• Assay Range: 0.05 to 10 ng/mL
• Assay Sensitivity: 0.05 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4854
• Gene Symbol: Notch3
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: NOTCH3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins