Gibco™ Human p16-INK4a Recombinant Protein, PeproTech®

Description

Recombinant Protein

110-02-1MG will be provided as 10 x 100 μg (110-02-100UG). 110-02-500UG will be provided as 5 x 100 μg (110-02-100UG). Recombinant Human p16-INK4a is a 16.5 kDa protein containing 156 amino acid residues. Kim, W.Y. and Sharpless, N.E. (2006)Cell 127(2):265-75. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

Specifications

• Accession Number: P42771, Q8N726
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 1029
• Molecular Weight (g/mol): 16.5 kDa
• Name: Human p16-INK4a
• Quantity: 100 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: Alternative reading frame; Arf; ARF-INK4a; CDK4 inhibitor p16 INK4; CDK4 inhibitor p16-INK4; CDK4I; CDKN2; Cdkn2a; cdkn2a {ECO:0000312; cell cycle inhibitor; cell cycle negative regulator beta; cell cycle regulator; CMM2; Cyclin dependent kinase 4 inhibitor A; cyclin dependent kinase inhibitor 2 A; Cyclin dependent kinase inhibitor 2 A (p16, inhibits CDK4); cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2 A; cyclin-dependent kinase inhibitor 2 A (melanoma, p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2 A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2 A p16Ink4a; cyclin-dependent kinase inhibitor 2 A p19Arf; cyclin-dependent kinase inhibitor 2 A, isoform 1; cyclin-dependent kinase inhibitor 2 A, isoform 2; cyclin-dependent kinase inhibitor 2 A, isoform 3; cyclin-dependent kinase inhibitor 2 A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; EMBL:AAL76336.1, ECO:0000312; EMBL:AAL76338.1, ECO:0000312; HGNC:1787; inhibits CDK4; INK4; INK4A; Ink4a/Arf; INK4a-ARF; isoforms 1/2/3; Melanoma p16 inhibits CDK4; mitochondrial smARF; MLM; MTS1; MTS-1; Multiple tumor suppressor 1; P14; P14 P16; P14ARF; p16; p16(INK4a); p16Cdkn2a; P16INK4; p16-INK4; p16INK4a; p16-INK4a; P19; P19 TP16; P19ARF; Pctr1; RGD:2323}; TP16; Tumor suppressor ARF
• Common Name: p16INK4a
• Gene Symbol: CDKN2A
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MEPAAGSSME PSADWLATAA ARGRVEEVRA LLEAGALPNA PNSYGRRPIQ VMMMGSARVA ELLLLHGAEP NCADPATLTR PVHDAAREGF LDTLVVLHRA GARLDVRDAW GRLPVDLAEE LGHRDVARYL RAAAGGTRGS NHARIDAAEG PSDIPD
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.