Invitrogen™ Human PCSK9 (NARC1) His-tag Recombinant Protein

Catalog No.	Quantity
PIRP88142	500 μg
PIRP88141	50 μg

Catalog No. PIRP88142 Supplier Invitrogen™ Supplier No. RP88142

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (500 μL) be added to the vial to prepare a stock solution of 1.11 mg/mL. Concentration is measured by UV.

PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. The protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

Specifications

• Accession Number: Q8NBP7
• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 255738
• Molecular Weight (g/mol): 72.5 kDa
• Name: Human PCSK9 (NARC1) His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 500 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: AI415265; AI747682; convertase subtilisin; convertase subtilisin/kexin type 9 preproprotein; FH3; HCHOLA3; hypercholesterolemia; LDLCQ1; NARC1; NARC-1; neural apoptosis regulated convertase 1; neural apoptosis-regulated convertase 1; OTTHUMP00000009833; PC9; PCSK9; Proprotein convertase 9; proprotein convertase PC9; proprotein convertase subtilisin/kexin type 9; PSEC0052; Subtilisin/kexin-like protease PC9
• Common Name: PCSK9
• Gene Symbol: PCSK9
• Biological Activity: Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag on ProA Biosensor, can bind Recombinant Human PCSK9 Protein, His Tag with an affinity constant of 16 nM as determined in BLI assay. Immobilized Anti-PCSK9(Amg)-IgG2 Antibody (at 2 μg/mL (100 μL/well) can bind Recombinant Human PCSK9 / NARC1 Protein (His Tag), the EC50 is 14-52 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human PCSK9, amino acids Met1-Gln692 (Accession # NP_777596.2) with a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag on ProA Biosensor, can bind Recombinant Human PCSK9 Protein, His Tag with an affinity constant of 16nM as determined in BLI assay. Immobilized Anti-PCSK9(Amg)-IgG2 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Human PCSK9 / NARC1 Protein (His Tag), the EC50 is 14-52 ng/mL.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.