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Human PD-1 Antibody, R&D Systems™
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Catalog No. AF1086SP
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25 μg
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AF1086SP 25 μg
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Description

Goat Polyclonal Antibody has been used in 26 publications

PD-1 Polyclonal specifically detects PD-1 in Cercocebus atys (Sooty Mangabey)∣Chinese Hamster∣Feline∣Human∣Mouse∣Primate∣Rhesus Macaque samples. It is validated for Bioassay∣Blockade of Receptor-ligand Interaction∣CyTOF∣Dual RNAScope ISH-IHC Compatible∣ELISA Capture (Matched Antibody Pair)∣ELISA Development∣Flow Cytometry∣Functional Assay∣Immunocytochemistry∣Immunohistochemistry∣Immunohistochemistry (Paraffin)∣Microarray∣Neutralization∣Surface Plasmon Resonance (SPR)∣Western Blot.

Specifications

Antigen PD-1
Applications Bioassay, Blockade of Receptor-ligand Interaction, CyTOF, Dual RNAScope ISH-IHC, ELISA Capture (Matched Antibody Pair), ELISA Development, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin), Microarray, Neutralization, Surface Plasmon Resonance, Western Blot
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 0.5-2 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, Blockade of Receptor-ligand Interaction, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. Q8IX89
Gene Alias CD279, CD279 antigen, hPD-1, PD1, PD1hPD-l, PDCD1, programmed cell death 1, programmed cell death protein 1, Protein PD-1, SLEB2
Gene Symbols PDCD1
Host Species Goat
Immunogen Mouse myeloma cell line NS0-derived recombinant human PD-1 Leu25-Gln167 Accession # Q8IX89
Purification Method Affinity Purified
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 5133
Test Specificity Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD-1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA-4 is observed.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Sooty Mangabey, Chinese Hamster, Feline, Human, Mouse, Primate, Rhesus Macaque
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Product Type Antibody
Isotype IgG
Product Line R&D Systems
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