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Human PD-1 Antibody, R&D Systems™
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Catalog No. AF1086SP
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25 μg
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AF1086SP 25 μg
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Description

Goat Polyclonal Antibody has been used in 26 publications

PD-1 Polyclonal specifically detects PD-1 in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Blockade of Receptor-ligand Interaction, CyTOF-ready, ELISA Capture (Matched Antibody Pair).

Specifications

Antigen PD-1
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Blockade of Receptor-ligand Interaction, CyTOF
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 0.5-2 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, Blockade of Receptor-ligand Interaction, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. Q8IX89
Gene Alias CD279, CD279 antigen, hPD-1, PD1, PD1hPD-l, PDCD1, programmed cell death 1, programmed cell death protein 1, Protein PD-1, SLEB2
Gene Symbols PDCD1
Host Species Goat
Immunogen Mouse myeloma cell line NS0-derived recombinant human PD-1 Leu25-Gln167 Accession # Q8IX89
Purification Method Affinity Purified
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 5133
Test Specificity Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD-1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA-4 is observed.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Human
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype IgG
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