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Gibco™ Human PDGF-BB Recombinant Protein

Catalog No. PHG0044
Encompass_Preferred
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Quantity:
1 mg
10 μg
100 μg
5 μg
50 μg

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in 100mM acetic acid containing 0.1% BSA to 0.1-1.0 mg/mL to regain full activity. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA. Storage: Store the lyophilized protein at 2-8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01127
For Use With (Application) Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
Formulation protein with no preservative
Gene ID (Entrez) 5155
Molecular Weight (g/mol) 25.4 kDa
Name Human PDGF-BB
Purification Method Sequential Chromatography
Quantity 5 μg
Endotoxin Concentration <0.1 ng/ μg
Gene Alias becaplermin; c-sis; FLJ12858; H-PDGF-BB; IBGC5; PDGF; pdgf protein; PDGF subunit B; PDGF, B chain; PDGF2; PDGF-2; PDGFB; PDGF-B; platelet derived growth factor subunit B; platelet derived growth factor, B polypeptide; platelet-derived growth factor 2; platelet-derived growth factor B; platelet-derived growth factor B chain; platelet-derived growth factor beta; platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); Platelet-derived growth factor, same as Sis (Simian sarcoma viral oncogene homologue, c-sis); proto-oncogene c-Sis; Sis; SSV
Common Name PDGF-BB
Biological Activity ED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of BALB/c 3T3 cells.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Sequence SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
Content And Storage 4°C
Classification Carrier-Free
Endotoxin Level <0.1 ng/μg
Shipping Condition Approved for shipment on Wet or Dry Ice
Expression System E. coli
Protein Family Growth Factors & Receptors
Protein Form Full Length, Recombinant, Ligand
Form Lyophilized
Protein Subtype PDGFs (Platelet-Derived Growth Factors)
Research Category Immunology, Neurobiology, Stem Cell Research, Angiogenesis, Oncology
Product Line Gibco™
Purity or Quality Grade >95% by SDS-PAGE
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