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Invitrogen™ Human PDGF-BB Recombinant Protein

Catalog No. PIRP8657
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10 μg
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Catalog No. Quantity
PIRP8657 10 μg
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Catalog No. PIRP8657 Supplier Invitrogen™ Supplier No. RP8657

Description

Recombinant Protein

PDGF-BB (human) Recombinant Protein, RP-8657, from Invitrogen.

Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-alpha, TGF-beta and EGF. Recombinant human PDGF-BB is a non-glycosylated, disulfide-linked homodimer, containing two 109 amino acid chains, with a total molecular weight of 24.3 kDa. Purity is typically greater than 95% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent proliferation of 3T3 indicator cells and is typically 1-3 ng/mL. The specific activity of this protein is 1x10^6 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability. Amino acid sequence: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIGIVRK KPIFKKATVT LGDHLACKCE TVAAARPVT.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01127
Concentration 0.1mg/mL
For Use With (Application) Control
Formulation 10mM sodium phosphate with no preservative; pH 7.5
Gene ID (Entrez) 5155
Molecular Weight (g/mol) ∽24.3
Name Human PDGF-BB
pH Range 7.5
Quantity 10 μg
Source E. coli
Storage Requirements -20°C, Avoid Freeze/Thaw Cycles
Regulatory Status RUO
Gene Alias H-PDGF-BB
Common Name Human PDGF-BB
Gene Symbol PDGFB
Specific Reactivity Human
Conjugate Unconjugated
Cross Reactivity Hu
Species E. coli
Recombinant Recombinant
Form Lyophilized
Protein Subtype Recombinant
Purity or Quality Grade purified
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in vitro research only.

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