Gibco™ Human PDGF-BB Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two beta chains (218 total amino acids). This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Specifications

• Accession Number: P01127
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 5155
• Molecular Weight (g/mol): 24.3 kDa
• Name: Human PDGF-BB
• Quantity: 100 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: becaplermin; c-sis; FLJ12858; H-PDGF-BB; IBGC5; PDGF; pdgf protein; PDGF subunit B; PDGF, B chain; PDGF2; PDGF-2; PDGFB; PDGF-B; platelet derived growth factor subunit B; platelet derived growth factor, B polypeptide; platelet-derived growth factor 2; platelet-derived growth factor B; platelet-derived growth factor B chain; platelet-derived growth factor beta; platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); Platelet-derived growth factor, same as Sis (Simian sarcoma viral oncogene homologue, c-sis); proto-oncogene c-Sis; Sis; SSV
• Common Name: PDGF-BB
• Gene Symbol: PDGFB
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells.The expected ED50 for this effect is 1.0-3.0 ng/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.