Abnova™ Human PDK3 (NP_004110.2, 546 a.a. - 993 a.a.) Partial Recombinant Protein with GST-tag expressed in Baculovirus infected Sf21 cells

Catalog No.	Quantity
89-944-920	5 μg

Catalog No. 89-944-920 Supplier Abnova™ Supplier No. P5713

Description

Used for Func, SDS-PAGE

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq]

Specifications

• Accession Number: NP_004110.2
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 2322
• Molecular Weight (g/mol): 77kDa
• Name: FLT3 (Human) Recombinant Protein
• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Purification Method: Glutathione sepharose chromatography
• Quality Control Testing: Loading 1 ug protein in SDS-PAGE
• Quantity: 5 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Regulatory Status: RUO
• Gene Alias: CD135/FLK2/STK1
• Common Name: FLT3
• Gene Symbol: FLT3
• Biological Activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
  Substrate : Srctide. ATP: 100 μM.
• Species: Baculovirus
• Recombinant: Recombinant
• Protein Tag: GST
• Expression System: Baculovirus infected insect cell (Sf21) expression system
• Form: Liquid
• Purity or Quality Grade: 98% by SDS-PAGE/CBB staining.