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Abnova™ Human PIK3CB (NP_006210.1, 1 a.a. - 1070 a.a.) and PIK3R1 (NP_852664.1, 1 a.a. - 724 a.a.) Full-length Recombinant Protein with GST-tag expressed in baculovirus infected Sf21 cells

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89-956-954 20μg
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Catalog No. 89-956-954 Supplier Abnova™ Supplier No. P5669
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Used for Func, SDS-PAGE

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]

Specifications

Accession Number NP_006210.1 (Gene ID : 5291);NP_852664.1 (Gene ID : 5295)
For Use With (Application) Functional Study, SDS-PAGE
Formulation Liquid
Gene ID (Entrez) 5291/5295
Molecular Weight (g/mol) 150kDa
Name PIK3CB/PIK3R1 (Human) Recombinant Protein
Preparation Method Baculovirus infected insect cell (Sf21) expression system
Purification Method Glutathione sepharose chromatography
Quality Control Testing Loading 1 ug protein in SDS-PAGE
Quantity 20 ug
Storage Requirements Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Regulatory Status RUO
Gene Alias DKFZp779K1237/MGC133043/PI3K/PI3KCB/PI3Kbeta/PIK3C1/p110-BETA
Common Name PIK3CB
Gene Symbol PIK3CB
Biological Activity The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Phosphatidylinositol. ATP: 100 uM.
Species Baculovirus
Recombinant Recombinant
Protein Tag GST
Expression System Baculovirus infected insect cell (Sf21) expression system
Form Liquid
Purity or Quality Grade 96% by SDS-PAGE/CBB staining
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