Invitrogen™ Human PMN (Neutrophil) Elastase ELISA Kit

Description

ELISA

The Human PMN (Neutrophil) Elastase) ELISA quantitates Hu PMN (Neutrophil) Elastase in human plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant PMN (Neutrophil) Elastase. Principle of the method The Human PMN (Neutrophil) Elastase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.

Specifications

• Accession Number: P08246
• Assay Range: 0.16 to 10.0 ng/mL
• Assay Sensitivity: 1.98 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cerebrospinal Fluid, Lavage Fluid, Plasma, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1991
• Gene Symbol: ELA2,GE,HLE,HNE,NE,PMN-E,SCN1
• Interassay CV: 5.6%
• Intraassay CV: 4.8
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cerebrospinal Fluid 10 μL, Lavage Fluid 10 μL, Plasma 10 μL, Supernatant 10 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Elastase-2, leukocyte elastase,neutrophil elastase
• Target: Neutrophil elastase
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 20 mins