Abnova™ Human PRKAA1 (NP_006242.4, 1 a.a.-550 a.a.)/PRKAB1 (NP_006244.2, 1 a.a. - 270 a.a.)/PRKAG1 (NP_002724.1, 1 a.a. - 331 a.a.) Full length Recombinant Protein with GST-tag expressed in Baculovirus infected Sf21 cells

Catalog No.	Quantity
89-958-104	5 μg

Catalog No. 89-958-104 Supplier Abnova™ Supplier No. P5780

Description

Used for Func, SDS-PAGE

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Specifications

• Accession Number: NP_006242.4 (Gene ID : 5562); NP_006244.2 (Gene ID : 5564); NP_002724.1 (Gene ID : 5571)
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 5562/5564/5571
• Molecular Weight (g/mol): 90kDa
• Name: PRKAA1/PRKAB1/PRKAG1 (Human) Recombinant Protein
• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Purification Method: Glutathione sepharose chromatography
• Quality Control Testing: SDS-PAGE Stained with Coomassie Blue
• Quantity: 5 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Regulatory Status: RUO
• Gene Alias: AMPK/AMPKa1/MGC33776/MGC57364
• Common Name: PRKAA1
• Gene Symbol: PRKAA1
• Biological Activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
  Substrate : SAMS peptide. ATP: 100 μM.
• Species: Baculovirus
• Recombinant: Recombinant
• Protein Tag: GST-His
• Expression System: Baculovirus infected insect cell (Sf21) expression system
• Form: Liquid
• Purity or Quality Grade: 81% by SDS-PAGE/CBB staining