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Abnova™ Human PRKAA2 (NP_006243.2, 1 a.a. - 552 a.a.) and PRKAB1(NP_006244.2, 1 a.a. - 270 a.a.) and PRKAG1 (NP_002724.1, 1 a.a. - 331 a.a.) Full-length Recombinant Protein with GST-tag expressed in baculovirus infected Sf21 cells

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89-958-109 5 μg
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Catalog No. 89-958-109 Supplier Abnova™ Supplier No. P5493
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Description

Used for Func, SDS-PAGE

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Specifications

Accession Number NP_006243.2 (Gene ID : 5563);NP_006244.2 (Gene ID : 5564);NP_002724.1 (Gene ID : 5571)
For Use With (Application) Functional Study, SDS-PAGE
Formulation Liquid
Gene ID (Entrez) 5563/5564/5571
Molecular Weight (g/mol) 89kDa
Name PRKAA2/PRKAB1/PRKAG1 (Human) Recombinant Protein
Preparation Method Baculovirus infected insect cell (Sf21) expression system
Purification Method Glutathione sepharose chromatography
Quality Control Testing Loading 1 ug protein in SDS-PAGE
Quantity 5 μg
Storage Requirements Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Regulatory Status RUO
Gene Alias AMPK/AMPK2/PRKAA
Common Name PRKAA2
Gene Symbol PRKAA2
Biological Activity The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: SAMS peptide. ATP: 100 uM.
Species Baculovirus
Recombinant Recombinant
Protein Tag GST
Expression System Baculovirus infected insect cell (Sf21) expression system
Form Liquid
Purity or Quality Grade 72% by SDS-PAGE/CBB staining
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