Invitrogen™ Human R-Spondin 1 (aa 1-146) His-tag Recombinant Protein

Catalog No.	Quantity
PIRP88158	1 mg
PIRP88157	100 μg

Catalog No. PIRP88158 Supplier Invitrogen™ Supplier No. RP88158

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (4000 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.

Specifications

• Accession Number: Q2MKA7
• For Use With (Application): Control, ELISA Standard, Binding Assay, Bioactivity
• Format: Lyophilized
• Formulation: PBS with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 284654
• Molecular Weight (g/mol): 23 kDa
• Name: Human R-Spondin 1 (aa 1-146) His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: CRISTIN3; cristin-3; cysteine-rich and single thrombospondin domain-containing protein 3; FLJ40906; hRspo1; mCristin-3; RGD1565558; Roof plate-specific spondin-1; RP11-566C13.1; RSPO; RSPO1; Rspondin; R-spondin; R-spondin 1; R-spondin homolog; R-spondin homolog (Xenopus laevis); R-spondin-1; thrombospondin type 1 domain containing gene Rspondin
• Common Name: RSPO1
• Gene Symbol: RSPO1
• Biological Activity: 1. Measured by its ability to bind recombinant mouse CD36 in a functional ELISA. 2. Measured by its ability to induce activation of beta-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.5 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Met1-Ala146
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: 1. Measured by its ability to bind recombinant mouse CD36 in a functional ELISA. 2. Measured by its ability to induce activation of beta-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.5 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >98% as determined by SDS-PAGE.