Gibco™ Human R-Spondin-2 Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human R-Spondin-2 is a 24.4 kDa protein consisting of 212 amino acid residues. Due to glycosylation, R-Spondin-2 migrates at an apparent molecular weight of approximately 30.0 kDa by SDS PAGE analysis under reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally-related, secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenin. This activity effectively activates and increases the subsequent nuclear signaling of beta-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/beta-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations.

Specifications

• Accession Number: Q6UXX9
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 340419
• Molecular Weight (g/mol): 24.4 kDa
• Name: Human R-Spondin-2
• Quantity: 100 μg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: 2610028F08Rik; AA673245; CRISTIN2; cristin-2; cysteine-rich and single thrombospondin domain-containing protein 2; D430027K22; ftls; hRspo2; mCristin-2; RGD1562331; Roof plate-specific spondin-2; Rspo2; R-spondin 2; R-spondin 2 homolog; R-spondin 2 homolog (Xenopus laevis); R-spondin-2; UNQ9384/PRO34209
• Common Name: RSPO2
• Gene Symbol: RSPO2
• Biological Activity: R-Spondin-2 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED50 for this effect is 0.8 -2.0 ug/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: ASYVSNPICK GCLSCSKDNG CSRCQQKLFF FLRREGMRQY GECLHSCPSG YYGHRAPDMN RCARCRIENC DSCFSKDFCT KCKVGFYLHR GRCFDECPDG FAPLEETMEC VEGCEVGHWS EWGTCSRNNR TCGFKWGLET RTRQIVKKPV KDTILCPTIA ESRRCKMTMR HCPGGKRTPK AKEKRNKKKK RKLIERAQEQ HSVFLATDRA NQ
• Content And Storage: -20°C
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.