Abnova™ Human RAF1 (NM_002880.2, 306 a.a. - 648 a.a.) Y340D and Y341D mutant Partial Protein expressed in Sf9 cells

Catalog No.	Quantity
89-959-278	100 μg

Catalog No. 89-959-278 Supplier Abnova™ Supplier No. P4836

Description

Used for Func, SDS-PAGE

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq]

Specifications

• Accession Number: NM_002880.2
• Concentration: 0.334 ug/uL
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 5894
• Molecular Weight (g/mol): 38.472kDa
• Name: RAF1 (Y340D/Y341D) (Human) Recombinant Protein
• Preparation Method: Insect cell (Sf9) expression system
• Purification Method: GST affinity chromatography
• Quality Control Testing: 2 ug/lane SDS-PAGE Stained with Coomassie Blue
• Quantity: 100 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing
• Regulatory Status: RUO
• Gene Alias: CRAF/NS5/Raf-1/c-Raf
• Common Name: RAF1
• Gene Symbol: RAF1
• Biological Activity: 189pmol/μg x min
• Species: Baculovirus
• Protein Tag: None
• Expression System: Insect cell (Sf9) expression system
• Form: Liquid