Invitrogen™ Human RANTES Uncoated ELISA Kit

ELISA

Supplier:  Invitrogen™ 885028788

Catalog No. 88-502-8788

Description

The Human RANTES Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human RANTES. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

Specifications

• Accession Number: beta-chemokine RANTES; CC chemokine; C-C motif chemokine 5; C-C motif chemokine ligand 5; CCL5; chemokine (C-C motif) ligand 5; D17S136E; eoCP; Eosinophil chemotactic cytokine; MGC17164; MuRantes; OTTHUMP00000197106; RANTES; RANTES protein; RANTES(3-68); RANTES(4-68); regulated upon activation normal T-cell expressed and secreted; regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; SIS d; SISd; SIS-delta; SISd; small inducible cytokine A5; small inducible cytokine subfamily A (Cys-Cys), member 5; small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228; Unknown (protein for MGC:127014)
• Assay Sensitivity: <46.9 pg/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CCL5
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hrs 45 mins

• Assay Range: 46.9 to 3000 pg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): 6352
• Kit Contents: Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, Biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, Assay Buffer A, Enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X), Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Target: CCL5 (RANTES)
• Total Assay Time: 24 hrs 30 mins