Invitrogen™ CD44v6 (Soluble) Human Instant ELISA™ Kit

Catalog No.	Quantity
50-179-95	128 Tests

Catalog No. 50-179-95 Supplier Invitrogen™ Supplier No. BMS210INST

Description

Includes

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD44var (v6), HRP-Conjugate (human sCD44var (v6) monoclonal antibody) and Sample Diluent, lyophilized
• Aluminum pouch(es) with a human sCD44var (v6) Standard curve (coloured)
• Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
• Sample Diluent
• Substrate Solution (tetramethyl-benzidine)
• Stop Solution (1M Phosphoric acid)
• Adhesive Films

Instant ELISA Kit

The Human Soluble CD44var (v6) ELISA quantitates Hu sCD44var (v6) in human serum, plasma, cell culture supernatants, urine or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sCD44var (v6). Principle of the method The Human sCD44var (v6) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

Specifications

• Accession Number: P16070
• Assay Range: 0.16-10.0 ng/mL
• Assay Sensitivity: 0.06 ng/mL
• Conjugate: HRP
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant, Urine
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 960
• Gene Symbol: CDW44,CSPG8,ECMR-III,HCELL,HUTCH-I,IN,LHR,MC56,MDU2,MDU3,MIC4,Pgp1
• Interassay CV: 10%
• Intraassay CV: 8.5%
• Kit Contents: Pre-coated 96 well Microplate, Detection Antibody, Anti-IgG-HRP, Sample Diluent, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers, lyophilized
• Label or Dye: HRP
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL; Urine,10 μL
• Storage Requirements: -20°C
• Synonym: VFF-7 Phagocytic glycoprotein I, Hermes antigen
• Target: Human
• Test Time: 20 min.
• Total Assay Time: 3 hr. 10 min.

For Research Use Only