Invitrogen™ Human Serpin A3/Alpha-1-Antichymotrypsin ELISA Kit

Description

ELISA

Human Serpin A3/Alpha-1-Antichymotrypsin quantitates human Serpin A3/Alpha-1-Antichymotrypsin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Serpin A3/Alpha-1-Antichymotrypsin.

SERPINA encodes Alpha 1-ACT. It is an early-stage acute-phase plasma protein and a serpin that preferentially inactivates chymotrypsin, cathepsin G, and chymase. Alpha-1-ACT, a serine protease inhibitor, is tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain. Regulation of the serine proteases and serine protease inhibitors plays an important role in neuromuscular differentiation. Prostate specific antigen (PSA), a chymotrypsin-like serine protease, is predominantly complexed to Alpha-1-ACT.

Specifications

• Accession Number: AACT; ACT; Alpha-1-antichymotrypsin; Alpha-1-antichymotrypsin His-Pro-less; Cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; growth-inhibiting protein 24; growth-inhibiting protein 25; MGC88254; serine (or cysteine) proteinase inhibitor, clade A, member 3; serpin A3; serpin family A member 3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3; SERPINA3
• Assay Range: 0.137 to 100 ng/mL
• Assay Sensitivity: 0.14 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 12
• Gene Symbol: SERPINA3
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: SERPINA3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins