Invitrogen™ Human SMAD4 ELISA Kit

Catalog No.	Quantity
EH429RBX5	5 x 96 Tests
EH429RB	96 Tests

Catalog No. EH429RBX5 Supplier Invitrogen™ Supplier No. EH429RBX5

Description

ELISA

Human SMAD4 quantitates human SMAD4 in supernatant. The assay will exclusively recognize both natural and recombinant human SMAD4.

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

Specifications

• Accession Number: AW743858; D18Wsu70e; deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; deletion target in pancreatic carcinoma 4 homolog; DPC4; hSMAD4; JIP; MAD (mothers against decapentaplegic Drosophila) homolog 4; MAD homolog 4; MADH4; Mothers against decapentaplegic homolog 4; mothers against decapentaplegic, Drosophila, homolog of, 4; mothers against DPP homolog 4; MYHRS; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4; SMAD4
• Assay Range: 1.3 to 300 ng/mL
• Assay Sensitivity: 1.3 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4089
• Gene Symbol: SMAD4
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: SMAD4
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov