Abnova™ Human SPHK1 (NP_001136074.1, 1 a.a. - 384 a.a.) Full-length Recombinant Protein with GST-tag expressed in baculovirus infected Sf21 cells

Catalog No.	Quantity
89-963-039	5 μg

Catalog No. 89-963-039 Supplier Abnova™ Supplier No. P5670

Description

Used for Func, SDS-PAGE

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Specifications

• Accession Number: NP_001136074.1
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 8877
• Molecular Weight (g/mol): 69kDa
• Name: SPHK1 (Human) Recombinant Protein
• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Purification Method: Glutathione sepharose chromatography
• Quality Control Testing: Loading 1 ug protein in SDS-PAGE
• Quantity: 5 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Regulatory Status: RUO
• Gene Alias: SPHK
• Common Name: SPHK1
• Gene Symbol: SPHK1
• Biological Activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Sphingosine. ATP: 100 uM.
• Species: Baculovirus
• Recombinant: Recombinant
• Protein Tag: GST
• Expression System: Baculovirus infected insect cell (Sf21) expression system
• Form: Liquid
• Purity or Quality Grade: 91% by SDS-PAGE/CBB staining