ProSci Human Spleen Lysate

Catalog No.	Quantity
16-595-634	0.1 mg

Catalog No. 16-595-634 Supplier ProSci Supplier No. RF10001030100

Description

Total protein normal spleen - Human

• Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin
• Tissue and cell debris was removed by centrifugation
• Protein concentration was determined with Bio-Rad protein assay
• The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Specifications

• For Use With (Application): Western Blot
• Tissue: Spleen
• Quantity: 0.1 mg
• Tissue Condition: Normal
• Host Species: Human
• Concentration: 2 mg/mL
• Content And Storage: Store at 2°C to 8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
• Protein State: Denatured